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. 2013 Jun 7;288(31):22777–22789. doi: 10.1074/jbc.M113.475467

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 9. — Redox titrations of GAPC1 in the presence of various [GSH]/[GSNO] or [GSH]/[GSSG] ratios. Reduced GAPC1 was incubated in the presence of varying [GSH]/[GSNO] (light gray bars) or [GSH]/[GSSG] (dark gray bars) ratios at a total concentration of 2 mm. After equilibrium in the redox buffers was reached (30 min), the remaining NADH-dependent activity was measured. Data are represented as mean percentage ± S.D. (n = 3) of the initial activity of reduced GAPC1 measured before the treatments.