TABLE 1.
Kinetic parameters for glycosylated substrate 1 and nonglycosylated substrate 4 substrate hydrolysis by ADAM10 and 17 constructs in presence and absence of l-galactose
The results are reported as averages ± S.D. (n = 3).
| Enzyme |
kcat/Km |
kcat |
Km |
|||
|---|---|---|---|---|---|---|
| Glycosylated | Nonglycosylated | Glycosylated | Nonglycosylated | Glycosylated | Nonglycosylated | |
| m−1 s−1 | s−1 | μm | ||||
| ADAM17 ECD | 7.6 ± 1.7 × 104a | 1.2 ± 0.1 × 104a | 0.25 ± 0.03a | 0.14 ± 0.01a | 3.0 ± 0.5a | 12 ± 3a |
| ADAM17 CD | 3.2 ± 0.02 × 104 | 1.7 ± 0.2 × 104 | 0.22 ± 0.03 | 0.07 ± 0.01 | 6.9 ± 1.3 | 4.0 ± 0.1 |
| ADAM17 ECD + l-gal | 2.3 ± 0.14 × 104 | 1.9 ± 0.13 × 104 | 0.08 ± 0.01 | 0.1 ± 0.01 | 3.2 ± 0.3 | 4.0 ± 0.3 |
| ADAM17 CD + l-gal | 3.6 ± 0.4 × 104 | 1.8 ± 0.14 × 104 | 0.36 ± 0.03 | 0.06 ± 0.03 | 10 ± 1.3 | 3.4 ± 0.9 |
| ADAM10 ECD | 0.8 ± 0.3 × 104a | 2.5 ± 0.7 × 104a | 0.06 ± 0.01a | 0.28 ± 0.03a | 8.5 ± 1.3a | 12 ± 3a |
| ADAM10 ECD + l-gal | 2.8 ± 0.00 × 104 | 0.9 ± 0.04 × 104 | 0.34 ± 0.02 | 0.02 ± 0.00 | 12 ± 1.6 | 2.0 ± 0.5 |
a Reported in Ref. 15.