TABLE 2.
Effect of noncatalytic domains on kinetic parameters of hydrolysis for glycosylated substrates 2 and 3 by ADAM10 and 17
The results are reported as averages ± S.D. (n = 3).
| Enzyme |
kcat/Km |
kcat |
Km |
|||
|---|---|---|---|---|---|---|
| Substrate 2 (73–82) | Substrate 3 (73–82) | Substrate 2 (73–82) | Substrate 3 (73–82) | Substrate 2 (73–82) | Substrate 3 (73–82) | |
| m−1 s−1 | s−1 | μm | ||||
| ADAM17 ECD | 6.1 ± 0.8 × 104 | 0.68 ± 0.06 × 104 | 0.42 ± 0.01 | 0.15 ± 0.03 | 7.6 ± 1.3 | 22 ± 6.0 |
| ADAM17 CD | 0.11 ± 0.01 × 104 | 0.12 ± 0.01 × 104 | 0.08 ± 0.01 | 0.06 ± 0.02 | 68 ± 11 | 50 ± 9 |