TABLE 3.
Effect of juxtamembrane and part of mature domain of TNFα on Km and kcat parameters for hydrolysis of substrates 4, 5, 6, and 7 by ADAM10 and 17
The results are reported as averages ± S.D. (n = 3).
| Enzyme |
kcat |
Km |
||||||
|---|---|---|---|---|---|---|---|---|
| Substrate 4 (72–81) | Substrate 5 (73–82) | Substrate 6 (57–82) | Substrate 7 (72–108) | Substrate 4 (72–81) | Substrate 5 (73–82) | Substrate 6 (57–82) | Substrate 7 (72–108) | |
| s−1 | μm | |||||||
| ADAM10 ECD | 0.28 ± 0.03 | 0.03 ± 0.04 | 0.06 ± 0.01 | 0.013 ± 0.001 | 12 ± 3 | 10 ± 1.2 | 13 ± 0.5 | 4.3 ± 1.5 |
| ADAM17 ECD | 0.14 ± 0.01 | 0.2 ± 0.02 | 0.34 ± 0.03 | 0.07 ± 0.005 | 12 ± 3 | 7.2 ± 0.6 | 24 ± 6.2 | 3.3 ± 0.2 |
| ADAM17 CD | 0.07 ± 0.01 | 0.05 ± 0.01 | 0.12 ± 0.03 | 0.03 ± 0.001 | 4.0 ± 0.1 | 16 ± 5 | 20 ± 0.2 | 4.3 ± 1.5 |