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. 2013 Oct 10;2:134. Originally published 2013 May 31. [Version 2] doi: 10.12688/f1000research.2-134.v2

Figure 2. FAM129B positively regulates Wnt/β-catenin signal transduction in a panel of three cell lines.

Figure 2.

( a) Immunoblots show three independent siRNAs reduce steady-state levels of endogenous FAM129B following 72 hr treatment with 20 nM siRNA. The beta-tubulin immunoblot serves as a control. Three independent siRNAs targeting FAM129B inhibit FAM129B expression in HT1080 (left), A2058 (middle), and A375 cells (right). ( b) FAM129B siRNA inhibit WNT3A-dependent luciferase reporter activity (BAR reporter) normalized to constitutively expressed Renilla luciferase in HT1080 (left), A2058 (middle), and A375 cells (right). ( c) FAM129B siRNA inhibit Wnt-dependent AXIN2 expression in HT1080 (left), A2058 (middle), and A375 cells (right) relative to beta-actin mRNA expression by qPCR. Columns and error bars represent mean and SEM, respectively. Data are representative of at least three separate biological replicates. *p<0.05 by unpaired, two-tailed T-test.