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. 2013 Sep 13;288(43):30990–31001. doi: 10.1074/jbc.M113.486456

FIGURE 3.

FIGURE 3.

Analysis of cell adhesion. A, adhesion of cells expressing Lu or B-CAM to LM-511 (top panel), LM-332 (center panel), and fibronectin (bottom panel). 96-well plates were coated with increasing concentrations of the proteins and incubated with control/HT1080F (●), Lu/HT1080F (▴) or B-CAM/HT1080F (■) at 37 °C for 1 h. Adherent cells were stained with Diff-Quik and counted. The control/HT1080F cells attached to LM-511 more readily than the Lu/HT1080F and B-CAM/HT1080F cells. Each point represents the mean of triplicate assays. Bars show S.D. *, p < 0.05 by Student's t test. B, inhibitory effects of mAb 87207 on adhesion of transfectants to LM-511. Cells preincubated with mAb 87207 and function-blocking antibodies against the indicated integrin subunits were added to LM-511-coated wells. After incubation for 30 min, the attached cells were stained and counted. Values are expressed as percentages of the number of cells adhering in the absence of antibody. Each column represents the mean of triplicate assays. Bars show S.D. *, p < 0.05 by Student's t test. mAb 87207 restored the adhesion of Lu/HT1080F and B-CAM/HT1080F cells to LM-511 in the presence of anti-integrin mAbs.