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Effect of different ligands on the conformation of PMCA. Specific incorporation of [125I]TID-PC/16 into PMCA was determined as described under “Experimental Procedures” in the presence of 2 mm EGTA (E2); 100 μm free Ca2+ (E1Ca); 50 μm (VO4)3− plus 2 mm EGTA (E2Vi); 50 μm (VO4)3− plus 100 μm free Ca2+ (E*CaVi); or 50 μm LaIII, 100 μm free Ca2+ plus 25 μm ATP (E1PCa·La). Specific incorporation of the probe in the presence of 2 mm EGTA (E2) was taken as 100%. Error bars, S.E.
