Skip to main content
. 2013 Aug 28;288(43):31080–31092. doi: 10.1074/jbc.M113.469189

FIGURE 2.

FIGURE 2.

Expression and transport function of two SNP variants of hGLUT2. A, expression of hGLUT2 wild type (WT) and two SNP variants (P68L and T110I) in mhAT3F cells. Cells transfected with a pCMV-hGLUT2-HA-IRES-hrGFP construct are identified by the expression of the GFP. Plasma membrane location of hGLUT2 (red in the left panels and white in the right panels) is revealed with an antibody to an extracellular epitope of hGLUT2 in nonpermeabilized cells. Nuclei are stained with DAPI (blue). Scale bar corresponds to 25 μm. B, Western blot analysis of membrane fractions from mhAT3F cells transfected or not transfected (NT) with different hGLUT2 constructs. hGLUT2 expression is revealed with an antibody to HA epitope. E-cadherin is used as a loading control of membrane fractions. C, membrane expression of WT, P68L, and T110I hGLUT2 in Xenopus oocytes injected with the corresponding cRNAs. D, dose-response curves of 2-DOG uptake by Xenopus oocytes injected with WT, P68L, or T110I hGLUT2 cRNA. Curves are fitted up using Michaelis-Menten nonlinear regression.