Skip to main content
. 2013 Aug 28;288(43):31080–31092. doi: 10.1074/jbc.M113.469189

FIGURE 4.

FIGURE 4.

Expression and transport functions of three engineered mutants of hGLUT2. A, membrane co-localization of wild type (WT), G20S, F295Y, and L368P hGLUT2 (red) and membrane marker E-cadherin (green) in infected mhAT3F cells analyzed by confocal microscopy. Nuclei are stained with DAPI (blue). Scale bar corresponds to 10 μm. B, similar migrating profiles of hGLUT2 WT and mutants (G20S, F295Y, and L368P) in membrane fractions of mhAT3F cells analyzed by Western blot. C, comparable membrane expression of WT, G20S, F295Y, and L368P hGLUT2 in Xenopus oocytes injected with the corresponding cRNAs. D, dose-response curves of 2-DOG uptake by Xenopus oocytes injected with WT, G20S, F295Y, and L368P hGLUT2. Curves are fitted up using Michaelis-Menten nonlinear regression.