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. 2013 Aug 28;288(43):31080–31092. doi: 10.1074/jbc.M113.469189

FIGURE 5.

FIGURE 5.

Impact of G20S, F295Y and L368P hGLUT2 mutants on insulin secretion. A, quantification by flow cytometry of plasma membrane expression for wild type (WT), G20S, F295Y, and L368P hGLUT2 in infected MIN6 cells that were cultured in 0 mm (light gray), 5 mm (dark gray), or 25 mm (black) glucose. Nonpermeabilized cells are labeled with an antibody to an extracellular epitope of hGLUT2. Noninfected cells (NI) are used as negative control. B and C, insulin mRNA levels (B) and protein contents (C) in MIN6 cells expressing WT, G20S, F295Y, or L368P hGLUT2 cultured in 25 mm glucose. D, glucose-induced insulin secretion by MIN6 cells expressing WT, G20S, F295Y, or L368P hGLUT2 in response to 1 mm (light gray), 5 mm (dark gray), or 25 mm (black) glucose. E, basal insulin secretion by MIN6 cells expressing WT, G20S, F295Y, or L368P hGLUT2 in the absence of glucose. ns, no significant differences between WT and mutants. *, p < 0.05; **, p < 0.01 comparison between WT and mutant hGLUT2 at the same glucose concentration.