FIGURE 6.

G20S, F295Y, and L368P hGLUT2 mutants increase pancreatic β cell differentiation. A, immunohistology analysis of rat pancreases cultured for 7 days in the absence (left panels) or presence (middle and right panels) of 10 mm glucose. Staining of insulin (red), amylase (green, left and middle panels), or glucagon (green, right panels) and nuclei (blue) in noninfected pancreas (NI), pancreas infected with an adenoviral vector encoding hGLUT2 wild type (WT), G20S, F295Y, or L368P. Scale bar corresponds to 100 μm. B–D, quantification of the surfaces occupied by insulin-positive (B), glucagon-positive (C), or amylase-positive (D) cells expressed as a percentage of total cell surface after 7 days of culture in 0 mm (white bars) or 10 mm glucose (gray bars) glucose. E, proliferative index of insulin-positive cells after 7 days of culture in 0 mm (white bars) or 10 mm (gray bars) glucose, as assayed by the frequency of BrdU-positive nuclei among insulin-positive cells. ##, p < 0.01. ns, nonsignificant, comparison between 10 and 0 mm glucose concentrations. **, p < 0.01 comparison between WT and mutant hGLUT2 at the same glucose concentration.