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. 2013 Aug 28;288(43):31080–31092. doi: 10.1074/jbc.M113.469189

FIGURE 7.

FIGURE 7.

Pancreatic β cells induced by G20S, F295Y, and L368P hGLUT2 mutants secrete insulin in response to glucose. A, glucose-induced insulin secretion by rat pancreases noninfected (NI) or infected with an adenoviral vector encoding hGLUT2 G20S, F295Y, or L368P. **, p < 0.01 comparison between NI and mutant hGLUT2 at the same glucose concentration. B and C, morphological studies by electron microscopy of rat pancreases noninfected (NI) or infected with an adenoviral vector coding G20S hGLUT2 mutant after 7 days in culture. Type A cells (very dense core granules) and type B cells (dense core granules with halo) are both visible (×3,400). Scale bar corresponds to 1 μm. D, immunogold staining of insulin producing cells in a rat pancreas infected with an adenoviral vector coding G20S hGLUT2 mutant. Most of the secretory granules show labeling on the amorphous dense cores (×16,000). Scale bar corresponds to 1 μm. E, measurement of the diameter of 600 granules of insulin producing cells, using images at a magnification of 12,500. ***, p < 0.001 comparison with NI.