FIGURE 8.

F295Y hGLUT2 mutant increases pancreatic β cell differentiation through ChREBP activation. A, immunohistology analysis of rat pancreases cultured for 7 days in the absence of glucose. Staining of insulin (red), amylase (green), and nuclei (blue) in pancreas infected with an adenoviral vector coding either a dominant-negative form of ChREBP (DN ChREBP), the F295Y hGLUT2 mutant, or both. Scale bar corresponds to 100 μm. B, quantification of the surfaces occupied by insulin-positive cells expressed as a percentage of total cell surface after 7 days of culture in 0 mm glucose. **, p < 0.01 comparison with NI.