FIGURE 9.
Suppression of C. rodentium virulence by regacin. C57BL/6 mice in groups of five were inoculated with 2 × 109 cfu of either C. rodentium wild-type strain, ICC169 (four groups), or its isogenic regA mutant (two groups). Regacin was administered perorally to one group of mice 15 min before inoculation with wild-type C. rodentium ICC169, to another group of mice 15 min before inoculation with the regA mutant, and 12 h after inoculation with C. rodentium ICC169 to a third group of mice. All mice in these three groups then received regacin twice daily for the duration of the experiment. Regacin was not given to the remaining two groups. Data are the mean ± S.D. cfu/g of feces from mice on days 1–5.