TABLE 1.
Strains and plasmids used in this study
NalR, nalidixic acid resistance; SmR, streptomycin resistance; CmR, chloramphenicol resistance; KnR, kanamycin resistance; TcR, tetracycline resistance; TpR, trimethoprim resistance.
| Strains/plasmids | Relevant characteristics | Source/reference |
|---|---|---|
| Strains | ||
| C. rodentium ICC169 | Spontaneous NalR derivative of wild-type C. rodentium ICC168, NalR | (19) |
| C. rodentium EMH1 | C. rodentium ICC169 regA::aphA-2, NalR, KnR | (17) |
| E. coli E22 | EPEC serotype O103:H2 | (20) |
| E. coli H10407 | ETEC serotype O78:H11, LT+, ST+ | (21) |
| E. coli 042 | EAEC serotype O44:H18, SmR, TetR, CmR | (22) |
| V. cholerae 395 | Serogroup O1, biotype Classical | (23) |
| E. coli MC4100 | F−araD139Δ (argF-lac)U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR thiA | (24) |
| E. coli JP8042 | ΔlacU169 recA56 tyrR366 | (25) |
| E. coli XL1-Red | endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac mutD5 mutS mutT::Tn10, TcR | Stratagene |
| Plasmids | ||
| pCR2.1-TOPO | High copy number vector, Apr, Kanr | Invitrogen |
| pACYC184 | Medium copy number vector, p15A ori, CmR, TcR | (26) |
| pACYC184-regA (pEH6) | regA in pACYC184, CmR | (17) |
| pACYC177-tyrR (pMU1065) | tyrR in pACYC177, KnR | (25) |
| kfc-lacZ (pMU2385-kfcC) | kfc promoter region in pMU2385, TpR | (27) |
| mtr-lacZ (pMU3190) | mtr promoter region in pMU2385, TpR | (28) |
| cooB-lacZ | cooB promoter region in pMU2385, TpR | This study |
| sefA-lacZ | sefA promoter region in pMU2385, TpR | This study |
| aap-lacZ | aap promoter region in pMU2385, TpR | This study |
| tcpA-lacZ | tcpA promoter region in pMU2385, TpR | This study |
| pACYC184-rns | rns in pACYC184, CmR | This study |
| pACYC184-regR | regR in pACYC184, CmR | This study |
| pACYC184-aggR | aggR in pACYC184, CmR | This study |
| pACYC184-toxT | toxT in pACYC184, CmR | This study |