Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Sep 6;288(43):31217–31228. doi: 10.1074/jbc.M113.491498

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 2. — SigT is degraded dependent on ClpP/SsrA during cell differentiation. A–C, ClpP protease is required for SigT degradation during cell differentiation. SigT protein dynamics of strains M145/pL86 (wild type + 3flag-sigT) and L45/pL86 (ΔclpP + 3flag-sigT) on R2YE medium covered with cellophane (A and B) or in a liquid R5-medium (C) for the times indicated as demonstrated by Western blot. Coomassie Blue staining of total protein served as a loading control. Spores of strains M145 (wild type, WT), M145/pL86 (WT + sigT) L45 (ΔclpP), L43 (ΔrstAΔclpP), L45/pL86 (ΔclpP + sigT), and L43/pL86 (ΔrstAΔclpP + sigT) were streaked on R2YE medium for 32 (D) or 45 h (E), respectively, and photographed. F, spores of strains M145 (wild type, WT), LM22 (ΔrstA), L45 (ΔclpP), and L43 (ΔrstAΔclpP) were streaked on R2YE medium for 60 h and photographed.