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. 2013 Sep 6;288(43):31217–31228. doi: 10.1074/jbc.M113.491498

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — A and B, RstA degradation independent of SigT during cell differentiation. RstA protein dynamics in strains M145/pLM36 (wild type + rstA-3flag) and LM21/pLM36 (ΔsigT + rstA-3flag) on R2YE medium (A) or in liquid R5-medium (B) for the times indicated as demonstrated by Western blot with α-FLAG antibody. C and D, RstA degradation independent of ClpP protease. RstA protein dynamics in strains M145/pL36 (wild type + rstA-3flag) and L45/pL36 (ΔclpP + rstA-3flag) on R2YE medium (C) or in liquid R5-medium (D) for the times indicated as demonstrated by Western blot. Coomassie Blue staining of total protein served as a loading control.