FIGURE 5.

NUB1L regulates the protein level of NEDD8 by interacting with P97. A, effect of NUB1L or its M5 mutant on the protein level of NEDD8. HA-NEDD8 was co-transfected with FLAG-tagged NUB1L or its M5 mutant into HEK 293T cells. The cell lysates were subjected to Western blotting using respective antibodies. B, as in A, in the presence of MG132. After transfection, the cells were treated with MG132 (DMSO as a control) for 12 h, and about 24 h after transfection, the cells were harvested and subjected to Western blotting. C, as in A and B, effect of NUB1L or its M5 mutant on the protein level of NEDD8ΔGG.