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Elastase activity in Mgp−/− animals. A and B, elastase activity in WT and Mgp−/− (KO) aortae from 4.5-week-old mice (A, n = 3) or in primary VSMC cultures (B, two animals from each genotype used to establish primary cultures) in the presence or absence of metalloproteinase inhibitor, EDTA, serine elastase inhibitor, PMSF, or ethanol (EtOH) vehicle control as indicated. C and D, expression of induced serine elastases in WT and KO aortae (C, n = 4) or primary VSMC cultures (D).
