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. 2013 Oct 25;7:194. doi: 10.3389/fncel.2013.00194

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Copyright © 2013 Bernis, Oksdath, Dupraz, Nieto Guil, Fernandez, Malchiodi, Rosso and Quiroga.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Wnt3a interaction with IGF-1r. (A) Growth cone particles were stimulated with Wnt3a and immunoprecipitated with a non-relevant primary antibody (IP control) or the αIR3 antibody to pull down IGF-1r. Blots were probed with an anti-Wnt3a antibody. Immunoprecipitation of αIR3 is shown to confirm successful IP and as a loading control. (B–D) Growth cone particles were incubated in control buffer or in the presence of 1.35 nM Wnt3a, immunoprecipitated with anti-Wnt3a and probed with (B) β gc antibody to reveal IGF-1r; (C) anti-Ror2; and (D) anti-TrkB (BDNF receptor prominent at the growth cone of developing neurons) Immunoprecipitation of Wnt3a is shown to confirm successful IP and as a loading control. IP, immunoprecipitate; S, soluble fraction after immunoprecipitation. All blots are representative of at least three independent experiments.