Figure 8.

(A) Wnt3a polarizing effect depends on the activation of Fz7. Double immunofluorescence micrographs of hippocampal neurons incubated for 24 h in the presence of 1.35 nM Wnt3a showing the distribution of the axonal marker Tau-1 and the neuronal marker β-III-tubulin. myc is a marker of transfection with the negative dominant form of Fz7-CRD. Scale bar: 20 mm. (B) Percentage (±sem) of neurons at different stages of differentiation grown for 20 h in medium containing 1.35 nM Wnt3a and transfected with pcDNA3.1-myc or Fz7-CRD (n = 3 independent experiments). At least 20 transfected cells were scored for each condition. ^*p < 0.01 compared with pcDNA3.1-myc.