Abstract
Two lines of experimental evidence indicate that the repeating ribosomal DNA (rDNA) genes of the yeast Saccharomyces cerevisiae are located on chromosome XII. First, the rDNA genes are linked mitotically to genes that have been previously mapped to chromosome XII. Second, yeast strains that have two copies of the chromosome containing the rDNA genes in every strain examined also have two copies of chromosome XII; this is not true for the other yeast chromosomes. These data also establish that in mitosis most of the rDNA genes in yeast are not extrachromosomal.
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- Byers B., Goetsch L. Electron microscopic observations on the meiotic karyotype of diploid and tetraploid Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1975 Dec;72(12):5056–5060. doi: 10.1073/pnas.72.12.5056. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cramer J. H., Farrelly F. W., Rownd R. H. Restriction endonuclease analysis of ribosomal DNA from Saccharomyces cerevisiae. Mol Gen Genet. 1976 Nov 17;148(3):233–241. doi: 10.1007/BF00332897. [DOI] [PubMed] [Google Scholar]
- Findly R. C., Gall J. G. Free ribosomal RNA genes in Paramecium are tandemly repeated. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3312–3316. doi: 10.1073/pnas.75.7.3312. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Finkelstein D. B., Blamire J., Marmur J. Location of ribosomal RNA cistrons in yeast. Nat New Biol. 1972 Dec 27;240(104):279–281. doi: 10.1038/newbio240279a0. [DOI] [PubMed] [Google Scholar]
- Kaback D. B., Bhargava M. M., Halvorson H. O. Letter: Location and arrangement of genes coding for ribosomal RNA in Saccharomyces cerevisiae. J Mol Biol. 1973 Oct 5;79(4):735–739. doi: 10.1016/0022-2836(73)90076-4. [DOI] [PubMed] [Google Scholar]
- Mortimer R. K., Hawthorne D. C. Genetic Mapping in Saccharomyces IV. Mapping of Temperature-Sensitive Genes and Use of Disomic Strains in Localizing Genes. Genetics. 1973 May;74(1):33–54. doi: 10.1093/genetics/74.1.33. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mortimer R. K., Hawthorne D. C. Genetic mapping in Saccharomyces. Genetics. 1966 Jan;53(1):165–173. doi: 10.1093/genetics/53.1.165. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nakai S., Mortimer R. K. Studies on the genetic mechanism of radiation-induced mitotic segregation in yeast. Mol Gen Genet. 1969;103(4):329–338. doi: 10.1007/BF00383483. [DOI] [PubMed] [Google Scholar]
- Parry E. M., Cox B. S. The tolerance of aneuploidy in yeast. Genet Res. 1970 Dec;16(3):333–340. doi: 10.1017/s0016672300002597. [DOI] [PubMed] [Google Scholar]
- Petes T. D., Botstein D. Simple Mendelian inheritance of the reiterated ribosomal DNA of yeast. Proc Natl Acad Sci U S A. 1977 Nov;74(11):5091–5095. doi: 10.1073/pnas.74.11.5091. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Petes T. D., Hereford L. M., Botstein D. Simple Mendelian inheritance of the repeating yeast ribosomal DNA genes. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 2):1201–1207. doi: 10.1101/sqb.1978.042.01.121. [DOI] [PubMed] [Google Scholar]
- Petes T. D., Hereford L. M., Skryabin K. G. Characterization of two types of yeast ribosomal DNA genes. J Bacteriol. 1978 Apr;134(1):295–305. doi: 10.1128/jb.134.1.295-305.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schweizer E., MacKechnie C., Halvorson H. O. The redundancy of ribosomal and transfer RNA genes in Saccharomyces cerevisiae. J Mol Biol. 1969 Mar 14;40(2):261–277. doi: 10.1016/0022-2836(69)90474-4. [DOI] [PubMed] [Google Scholar]
- Seligy V. L., James A. P. Multiplicity and distribution of rDNA cistrons among chromosome I and VII aneuploids of Saccharomyces cerevisiae. Exp Cell Res. 1977 Mar 1;105(1):63–72. doi: 10.1016/0014-4827(77)90152-5. [DOI] [PubMed] [Google Scholar]
- Shaffer B., Brearley I., Littlewood R., Fink G. R. A stable aneuploid of Saccharomyces cerevisiae. Genetics. 1971 Apr;67(4):483–495. doi: 10.1093/genetics/67.4.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Øyen T. B. Chromosome I as a possible site for some rRNA cistrons in Saccharomyces cerevisiae. FEBS Lett. 1973 Feb 15;30(1):53–56. doi: 10.1016/0014-5793(73)80617-9. [DOI] [PubMed] [Google Scholar]