Fig. 5.

Optical sections of fluorescently labeled microtubules in HUVEC cells imaged by RCM with sweep-factor M = 1 (A), which gives an image with standard diffraction limited resolution of a wide-field fluorescence microscope. By imaging in double-sweep mode (sweep-factor M = 2) (B) gives resolution improvement by a factor of √2. Measured diameter of the microtubules is reduced from 255 nm for M = 1 to 185 nm for M = 2. The insets show junctions of microtubules (C, D) and parallel microtubules (D, F) unresolved with wide-field resolution (C, D) that can be distinguished by RCM in double sweep mode (E, F). The full 3D image stack is shown in Media 1 ^{(4.7MB, AVI)} . Scale bars are 1 μm.