Fig. 3.

Hepatic neutrophil accumulation after Con A administration. Mice were treated as described in the legend to Figure 2 with vehicle/saline, TCDD/saline, vehicle/Con A, or TCDD/Con A. A, Mice were euthanized 4h after saline or Con A administration. Paraffin-embedded liver sections were stained for neutrophils. Representative liver sections were photographed at ×40 magnification. Examples of positive neutrophil staining are indicated by arrows. B, Immunohistochemical staining of neutrophils in the livers at 4h was quantified as described in Materials and Methods. a, p < 0.05 vehicle/Con A versus vehicle/saline. b, p < 0.05 TCDD/Con A versus TCDD/saline. Data represent the mean ± SEM of 3–4 independent replicates per treatment group.