Fig. 2.

Characterization of EphB1 expression in human glioma cell lines. (A) The relative mRNA expression levels of EphB1 (target mRNA/β-actin mRNA ratios) in the cells were calculated by qRT-PCR. The value of U251 was set at 1. Results shown in (A) are typical of at least 2 replicate experiments. (B) Immunoprecipitation (IP) using total cell lysate from each of the indicated cell lines was performed. Equal amounts of cell lysates were immunoprecipitated with anti-EphB1 antibody. The immunoprecipitates were probed by immunoblotting (IB) with the antibody indicated. PY, phosphotyrosine. Whole cell lysates (WCL) were also immunoblotted with EphB1 or β-actin antibody as a loading control for equal protein loading of the 3 cell lines.