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. 2013 Dec;15(12):1696–1709. doi: 10.1093/neuonc/not136

Fig. 2.

Fig. 2.

Real-time measurements of cell migration and proliferation in the MACC1-overexpressing GBM cell line U251. (A) The human GBM cell line U251 was stably transfected with pcDNA3.1/MACC1. Overexpression of MACC1 was determined by quantitative real-time RT-PCR as well as by Western blotting using MACC1- and V5-specific antibodies (vs U251/vector cells, respectively). (B) Anchorage-independent cell proliferation was analyzed by soft agar colony formation assay. MACC1-expressing cells showed a significant increase of formed colonies to 127% (vs U251/vector; P = .029). (C and D) Cell proliferation as determined by the xCELLigence system. Cell index values were monitored as described in Fig. 1. (C) Real-time proliferation curve of U251/vector and U251/MACC1 cells. (D) MACC1-expressing cells show a statistically significant increased proliferation rate (P = .046). (E and F) Cell migration as determined by the xCELLigence system. Cell index values were monitored as described in Fig. 1. (E) Real-time migration curve of U251/vector and U251/MACC1 cells. (F) MACC1-expressing cells show a statistically significant increased migration rate (P = .003).