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. Author manuscript; available in PMC: 2014 Oct 28.
Published in final edited form as: Dev Cell. 2013 Oct 10;27(2):10.1016/j.devcel.2013.09.008. doi: 10.1016/j.devcel.2013.09.008

Figure 6. LET-60 RAS functions downstream to DAF-2 and nutritional cues to regulate MPK-1 activation.

Figure 6

Dissected adult hermaphrodite germ lines stained for DNA (DAPI, white) and dpMPK-1 (red). A–F: let-60(ga89gf) (A–D) and daf-2;let-60(ga89gf) (E–F) animals fed or starved for 2 hours. A–D: At 25°C upon starvation (D) let-60(ga89gf) worms displays continuous dpMPK-1 through zone 1 (yellow line) 1 and produce double rows of small oocytes (arrow heads). At 20°C let-60(ga89gf) worms downregulate dpMPK-1 in zone 1 (B). E–F: daf-2;let-60(ga89gf) worms display continuous dpMPK-1 and multiple oocytes both on and off food at 25°C. Experiments performed 3 times; each time 40-germ lines analyzed. G–L: Quantitative measure of dpMPK-1 levels in zones 1 and 2 from germ lines of fed or starved DAF-2∷GFP (vizIs23) worms, taken with Image J. X-axis depicts germ cell position along the length of the germ line; Y-axis measures the dpMPK-1 levels as pixel intensity. Scale bar: 20µm