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. Author manuscript; available in PMC: 2014 Apr 3.
Published in final edited form as: Nature. 2013 Jun 23;502(7469):114–118. doi: 10.1038/nature12265

Figure 3. Na+ ions and Asp favor the outward facing state.

Figure 3

Populations of low- (top, green), intermediate- (center, red) and high- (bottom, blue) FRET states as a function of a, Na+ ions added in the presence of 10 μM Asp and b, Asp added in the presence of 2 mM Na+. The titrations yielded dissociation constants (KD s) of 1.6± 0.3 mM and 6.5± 2.5 μM and Hill coefficients of 1.3± 0.3 and 0.9 ± 0.3, respectively. Shown are averages and standard deviations from at least three independent datasets (each containing at least 250 molecules). Solid lines through the data points are the results of global fitting of the data to Hill equation.