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. 2013 Nov 4;4:43. doi: 10.1186/2040-2392-4-43

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Copyright © 2013 Nuytens et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Normal expression of actin and the proteins involved in dense granule formation and secretion in platelets of Nbea^+/-mice. (A) Western blot analysis detected no changes in the total actin levels in platelets of Nbea^+/- mice compared to Nbea^+/+ mice. Actin expression was normalized to the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) content and the expression in platelets of wild-type mice was set at 100% (n = 4/genotype). (B) The total amount of Munc13-4, Rab27b and Calmodulin was unaltered in platelets of Nbea^+/- mice. Protein levels were normalized to actin and the expression level in platelets of Nbea^+/+ mice was set at 100% (n = 4 samples/genotype).