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Effects of TGF-β1 on the proliferation of human NP cells. Treatment of human NP cells with different concentrations of TGF-β1 for nine days. The concentrations of TGF-β1 were 0.1, 1.0 and 10.0 ng/ml. Absorbance values were measured at 450 nm using a spectrophotometer and the results shown are from three independent experimental cultures. *P<0.05 and **P<0.01 compared with control. TGF-β1, transforming growth factor-β1; NP, nucleus polposus.
