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Cultivation of human periodontal ligament fibroblasts (HPLFs). (A) Fourth passage of HPLFs. Cells were either fusiform or dendroid in shape, with full cell bodies, clear nuclei, and two or three fine cytoplasmic processes. Original magnification, ×200. (B) Growth curve of HPLFs. The growth curve was similar to an upside down ‘S’ with the arrest, logarithmic growth, and plateau phases. The multiplication of HPLFs was completed within 35.6 h.
