Figure 4.

Influence sEPCR on the level of cytokines and interleukins in plasma. (A) We analyzed the correlation between the amount of sEPCR in plasma and a set of 100 biologically active plasma proteins. The membranes were incubated with serum from ovarian cancer patients (10X in PBS albumin 1%). Signal intensities were quantified with a Bio-Imaging System MF-ChemiBis 4.2 and analyzed with Multi Gauge V3.2 software. We compared the significance (r ≥0.60) of plasmatic sEPCR in group 1 (sEPCR <100, blue histograms) and in group 2 (sEPCR >100, red histograms). When plasma sEPCR is >100 ng/ml, the expression of Fas-Ligand, CD 40 ligand, bFGF, FGF-9, IGF-II, IGFbp 3 and 6, IL-ra (receptor antagonist), IL-8, IL-13ra, IL-17b, IL-17c, IL-17R, IL-28A, IL-29, MIP-1, MMP-7, MMP-9, MMP-10 (r ≥0.60) and VEGF (r= 0.40) not only reversed but also increased significantly. (B) The experimental data were further subjected to scrutiny using the Sprearman statistical test. These results confirmed the results shown in (A) and also revealed some additional ones between sEPCR levels and the recently classified type III interferon group.