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. 2013 Aug 7;43(4):1059–1065. doi: 10.3892/ijo.2013.2055

Figure 3.


Figure 3.

Cf-GP induces apoptosis of the AGS cells. Cells cultured in 6-well plates were treated with Cf-GP (5, 10 and 20 μ g/ml) and then collected in medium containing 1% FBS. The percentages of apoptotic and necrotic cells were determined using a Muse Annexin V and Dead Cell kit as described in the Materials and methods. Cells in the early stage of apoptosis are Annexin V-positive and 7-AAD-negative, and those in late apoptosis are Annexin V-positive and 7-AAD-positive.