Table 2. Endogenous CK levels (pmol g−1 fresh weight) in leaves and roots of T1 PHT::ZmCKX1 barley plants.
| PHT2 | WT | WT | PHT2 | WT | PHT2 | WT | PHT2 | WT | |
| 4-m-Old Roots | 3-m-Old Roots | 4-m-Old Roots | 14-d-Old Leaves | 14-d-Old Leaves | 1-m-Old Leaves | 1-m-Old Leaves | 4-m-Old Leaves | 4-m-Old Leaves | |
| tZ | *0.67±0.01 | 0.54±0.03 | 0.45±0.02 | *1.67±0.25 | 2.52±0.31 | *1.29±0.07 | 2.40±0.34 | *2.80±0.25 | 1.69±0.28 |
| tZR | *1.36±0.25 | 0.78±0.08 | 0.82±0.12 | *0.63±0.15 | 0.99±0.16 | *0.56±0.06 | 1.53±0.12 | *0.25±0.03 | 0.10±0.01 |
| tZOG | 0.30±0.04 | 0.40±0.02 | 0.41±0.08 | *4.65±0.42 | 6.97±0.55 | 6.08±1.19 | 8.14±0.76 | *3.53±0.40 | 2.32±0.38 |
| tZROG | *0.23±0.02 | 0.15±0.01 | 0.15±0.01 | *0.10±0.02 | 0.16±0.04 | *0.15±0.02 | 0.27±0.09 | 0.11±0.01 | 0.12±0.01 |
| tZ9G | *3.08±0.84 | 11.04±1.61 | 11.23±0.79 | *23.58±3.42 | 44.48±2.82 | *27.81±4.53 | 54.62±6.95 | 41.65±4.07 | 35.18±4.93 |
| tZR5′MP | *2.61±0.39 | 0.69±0.05 | 0.66±0.06 | *0.61±0.24 | 1.19±0.24 | *0.80±0.10 | 1.69±0.38 | *0.53±0.07 | 0.29±0.05 |
| Active | 2.03 | 1.32 | 1.27 | 2.30 | 3.51 | 1.85 | 3.93 | 3.05 | 1.79 |
| Total | 8.25 | 13.60 | 13.72 | 31.24 | 56.31 | 36.69 | 68.65 | 48.87 | 39.70 |
| cZ | 0.22±0.02 | 0.26±0.03 | 0.28±0.05 | *0.29±0.03 | 0.42±0.05 | 0.30±0.02 | 0.28±0.02 | 1.10±0.20 | 1.45±0.13 |
| cZR | 2.49±0.26 | 3.05±0.24 | 3.17±0.42 | *1.53±0.31 | 2.30±0.28 | 1.35±0.14 | 1.74±0.28 | 1.05±0.22 | 1.20±0.13 |
| cZOG | *1.90±0.17 | 3.43±0.42 | 3.20±0.24 | *13.23±1.49 | 18.36±0.37 | 15.28±0.82 | 12.32±1.56 | *32.12±4.76 | 22.27±2.68 |
| cZROG | 5.56±0.31 | 5.21±0.66 | 5.81±0.63 | 1.56±0.14 | 1.99±0.14 | 2.07±0.20 | 1.77±0.25 | 1.76±0.04 | 1.68±0.18 |
| cZ9G | *0.02±0.00 | 0.41±0.02 | 0.45±0.08 | *0.18±0.03 | 0.32±0.05 | *0.24±0.04 | 0.38±0.03 | 0.82±0.09 | 0.92±0.19 |
| cZR5′MP | 8.42±0.79 | 5.95±0.93 | 7.22±1.37 | *6.11±0.56 | 14.78±2.04 | *3.56±0.14 | 8.36±1.18 | *2.85±0.44 | 6.25±0.85 |
| Active | 2.71 | 3.31 | 3.45 | 1.82 | 2.72 | 1.65 | 2.02 | 2.15 | 2.65 |
| Total | 18.61 | 18.31 | 20.13 | 22.90 | 38.17 | 22.80 | 24.85 | 39.70 | 33.77 |
| DHZ | UDL | UDL | UDL | UDL | UDL | UDL | UDL | UDL | UDL |
| DHZR | 0.03±0.00 | 0.03±0.01 | 0.03±0.00 | UDL | UDL | UDL | UDL | UDL | UDL |
| DHZOG | 0.37±0.05 | 0.39±0.05 | 0.20±0.03 | *1.65±0.40 | 3.14±0.41 | *1.71±0.22 | 3.68±0.44 | 4.37±0.51 | 4.12±0.42 |
| DHZROG | 1.66±0.21 | 1.11±0.13 | 1.09±0.13 | *0.24±0.05 | *0.71±0.09 | 0.49±0.07 | *0.85±0.04 | *0.40±0.03 | 0.74±0.04 |
| DHZ9G | UDL | UDL | UDL | UDL | UDL | UDL | UDL | UDL | UDL |
| DHZR5′MP | UDL | UDL | UDL | UDL | UDL | UDL | UDL | UDL | UDL |
| Total | 2.06 | 1.53 | 1.32 | 1.89 | 3.85 | 2.20 | 4.53 | 4.77 | 4.86 |
| iP | *0.32±0.03 | 0.70±0.01 | 0.10±0.01 | 0.21±0.03 | 0.22±0.03 | 0.32±0.05 | 0.29±0.05 | 0.25±0.04 | 0.17±0.03 |
| iPR | 2.91±0.21 | 3.54±0.32 | 2.25±0.32 | *2.27±0.35 | 3.31±0.08 | 2.38±0.26 | 2.47±0.26 | *3.52±0.23 | 2.48±0.38 |
| iP9G | *0.25±0.04 | 2.03±0.07 | 1.40±0.04 | *0.39±0.06 | 0.91±0.15 | *0.35±0.06 | 0.93±0.16 | *4.71±0.74 | 1.93±0.23 |
| iPR5′MP | *5.54±0.38 | 2.90±0.40 | 2.09±0.06 | *1.60±0.15 | 2.74±0.09 | *2.18±0.16 | 2.83±0.18 | *2.12±2.72 | 0.65±0.90 |
| Active | 3.23 | 4.24 | 2.35 | 2.48 | 3.53 | 2.70 | 2.76 | 3.77 | 2.65 |
| Total | 9.02 | 9.17 | 5.84 | 4.47 | 7.18 | 5.23 | 6.52 | 10.60 | 5.23 |
The CKs analyzed included: free bases of trans-zeatin (tZ), cis-zeatin (cZ), dihydrozeatin (DHZ) and N 6-isopentenyladenine (iP); their N9-ribosides (tZR, cZR, DHZR, iPR); O-glucosides (tZOG, cZOG, DHZOG); O-glucoside-N 9-ribosides (tZROG, cZROG, DHZROG); N 9-glucosides (tZ9G, cZ9G, DHZ9G, iP9G); and N 9-riboside-5′-monophosphates (tZR5′MP, cZR5′MP, DHZ5′MP, iPR5′MP); sums of free bases and N 9-ribosides are considered to comprise the pool of active CKs; mean values with standard deviations obtained from three biological replicates are presented; asterisks indicate significant differences between control and transformed tissue according to Student's unpaired t-tests at P≤0.05; UDL – under detection limit; WT – plants germinated from wild-type grains.