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. 2013 Nov 15;8(11):e79648. doi: 10.1371/journal.pone.0079648

Figure 2. siRNA transfection effectively downregulated corresponding phosphatase RNA expression in astrocytes.

Figure 2

(a) Determination of the silencing effect of six genes: APP6, ALPL, DUSP11, PPP1CC, PTPN11 and PNKP. The siRNAs of the six genes (ACP6, ALPL, DUSP11, PPP1CC, PTPN11 and PNKP), were used to transfect astrocytes (50 nM). Total RNA was extracted at 48 h post transfection; a relative quantification method was used to calculate the target gene transcription. Data were analyzed using 2−ΔΔCt. β-actin was used as the internal control. Error bars represent the standard deviation from triplicate samples. * indicates P<0.05. (b,c) Relationship between transfection time and the silencing effect of the ALPL and PNKP genes. The siRNAs of the ALPL and PNKP genes were used to transfect astrocytes (50 nM), and the total RNA was extracted at 24, 48 and 72 hr post transfection; a relative quantification method was used to calculate the target gene transcription. Data were analyzed using 2−ΔΔCt. β-actin was used as the internal control. Error bars represent the standard deviation from triplicate samples. * indicates P<0.05.