Figure 4. Kupffer cells derived from BDL iNKT^−/− mice produce less NO. and more neutrophil chemoattractants.

Kupffer cells were isolated from experimental groups composed of 3-5 mice on day 3 post-BDL; total RNA was extracted and purified; iNOS mRNA expression was quantified (A). Additional purified Kupffer cells were transferred to half-area 96-well plates and cultured in the presence of LPS. NO₂, equivalent to NO^., (B) and TNF-α, MIP-2, and KC (C) in the culture supernatants were quantified. Data are the means ± SD derived from 5 wells in a single experiment representative of two independent experiments in which each group was composed of 3-5 mice. *Significantly different from Kupffer cells obtained from BDL WT mice; p<0.05 (Student’s t-test).