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. 2013 Nov 15;8(11):e80310. doi: 10.1371/journal.pone.0080310

Figure 6. Ectopical expression of cMIP and CAB063 in HeLa cells.

Figure 6

HeLa cells were transfected with pCI-CAB080 (a) and with pCI-CAB063 (b, c), respectively. The cells were analyzed by immunofluorescence microscopy using (A) Evans blue for cytoplasmic counterstaining, (B) anti-CAB080 mouse antibodies (a) and anti-CAB063 rabbit antibodies (b, c), respectively, and (C) DAPI to stain nuclei (c). Scale bar = 5 µm.

a, Enrichment of cMIP (CAB080) in the cytoplasm of a transfected HeLa cell located next to a not transfected cell. b, c: Ectopically expressed CAB063 localizes to the host cell nucleus showing an intensified rim around the nucleus. b and c show representative examples of transfected cells, fixed with PFA (b, showing a not transfected and two transfected cells) and methanol (c), respectively.