Table 3. Selection of immunoreactive C. abortus-proteins identified by 2D immunoblot analysis.
| Spot-no. | Gene symbol / Protein description | Locus tag | CalculatedMW (kDa) | pI | A | B | C | D | E | F | G | H | I | J | K | L | M | N | O |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 13 | CAB031 / hypothetical protein | CAB031 | 87.0 | 4.54 | +++ | - | + | - | + | + | ++ | +++ | + | - | - | - | - | - | - |
| 14 | tsf / elongation factor Ts | CAB046 | 30.7 | 5.42 | + | + | ++ | - | +++ | + | (+) | ++ | +++ | ++ | - | - | - | - | - |
| 28 | CAB063 / hypoth. protein | CAB063 | 54.0 | 7.75 | + | + | +++ | ++ | +++ | ++ | + | ++ | - | - | - | - | - | - | - |
| 15 | mip | CAB080 | 28.1 | 5.09 | (+) | + | + | - | +++ | +++ | ++ | +++ | +++ | ++ | - | - | (+) | - | - |
| 31 | mip / putative macrophage infectivity potentiator lipoprotein | CAB080 | 28.1 | 5.09 | (+) | + | + | - | + | +++ | + | +++ | +++ | ++ | - | - | - | - | - |
| 2 | rpoA | CAB113 | 43.5 | 5.62 | +++ | +++ | + | + | (+) | + | (+) | + | - | +++ | (+) | - | - | - | - |
| 36 | pgi | CAB703 | 57.9 | 5.4 | + | - | + | + | ++ | +++ | ++ | +++ | +++ | +++ | - | - | (+) | - | - |
| 9 | pmp18D | CAB776 | 163.0 | 5.27 | ++ | + | + | - | +++ | +++ | - | ++ | + | + | - | - | - | - | - |
| 26 | pmp18D / polymorphic outer membrane protein | CAB776 | 163.0 | 5.27 | + | +++ | ++ | - | ++ | +++ | ++ | +++ | +++ | +++ | - | - | - | - | - |
| 37 | CAB821 / hypothetical protein | CAB821 | 64.0 | 5.98 | + | - | + | - | +++ | +++ | +++ | + | (+) | + | - | - | - | - | - |
For sera (A – O) see Table 1. The signal intensities of reactive protein spots are presented semiquantitatively: (-), negative; (+), very weak, corresponding to the intensity of MIP (spot 15) in Figure 1d; + weak, corresponding to the intensity of MIP in Figure 1b; ++ moderate corresponding to the intensity of MIP in Figure 1c; +++ strong, corresponding to the intensity of MIP in Figure 1a. Column 4 shows the calculated molecular mass and column 5 the isoelectric point (pI) of the identified proteins. A list of all identified immunoreactive C. abortus-proteins is presented in Table S1.