TABLE I.
Selected data for microfluidic-based cell cultivation and perfusion systems.
| Cell type | Size of culture area | Medium flow rate | Shear stress (N/m2) | Ref. |
|---|---|---|---|---|
| Mouse myoblast | NA | 15 nl/min | NA | 61 |
| Hepatocytes | H: 100 μm | 0.5 ml/h | NA | 40 |
| Hepatocytes | 5 ml | 18 ml/min | <0.033 | 41 |
| Kidney Crandell feline fibroblast and immortalised mouse hippocampal | 750 mm3 | 24 μl/min | NA | 42 |
| Human hepatocellular carcinoma cells and mouse embryonic fibroblasts | D: 600 μm, H: 35 μm | 0.3 μl/min | 0.07 | 43 |
| Human epithelial cells, epithelial-like breast cancer cells, and colon carcinoma cells | Depends on cell types under investigation | 5 μm/s | 0.07 | 45 |
| HeLa cells | D: 1430 μm, H: 180 μm | NA | 10–5 | 46 |
| Rat neuronal cells and human breast cancer cells | D: 600 μm | 5 and 10 μl/min | NA | 47 |
| Hepatocytes | L: 1 cm, W: 200 μm, H: 100 μm | 90 μl/h | NA | 66 |
| Oral cancer cells | H: 180 μm | 9 μl/h | NA | 67 |
| T-lymphocytes | H: 250 μm | 75 μl/h | NA | 59 |
| Oral cancer cells | D: 900 μm, H: 220 μm | 8.5 μl/h | NA | 48 |
| Colorectal adenocarcinoma | D: 1 mm, H: 250 μm | 1.2–3.9 μl/h | NA | 49 |
| Primary articular chondrocytes | D: 2.2 mm, H: 400 μm | 1.5 μl/h | NA | 51 |
| Human mammary epithelial cell line | D: 2 mm | 3 and 5 μl/h | NA | 52 |
| Primary human hepatocyte | W: 100 μm | 5 μl/h each inlet | NA | 53 |
| Hepatocarcinoma | 40 μl | 0.1 and 25 μl/min | NA | 54 |
| HT-29 human carcinoma | D: 200 μm, H: 150 μm | 4.5 μl/min | 0.0013 | 69 |
| Murine embryonic stem cells and murine 3T3 fibroblasts | 0.32 μl | 10 μl/h65 μl/h | 0.01 | 80 |
| Human bone marrow mesenchymal stem cells | D: 3000 μm, H: 100 μm | 0.1 μl/min | NA | 90 |
| Human embryonic stem cells | H: 200 μm, W: 1 mm, L: 1 cm | 1.5 ml/h | 0.06 | 87 |
| Amniotic stem cells | 5 mm × 5 mm | 1.7 μl/min | NA | 93 |
| Human mesenchymal stem cells | 60 nl | 6.5 nl/s | 0.05 | 94 |
| Human mesenchymal stem cells | W: 500 μm, H: 53 μm, L: 2 cm | 0.02–0.1 μl/h | 0.006–0.3 | 91 |
| Human embryonic stem cells | D: 3 mm | 0.3 μl/min | NA | 89 |
| Human breast cancer cells | 2.5 mm × 1.25 mm | 20 μl/h | NA | 98 |
| Human breast cancer cells | L: 2000 μm, W: 1000 μm, H: 30 μm | 20 μl/h | NA | 99 |
| Neurite of dorsal root ganglia neurons | W: 250 μm | 0.2 μl/min | NA | 100 |
| Xenopus embryonic spinal neurons | 2 mm × 7 mm × 130 μm | 680 nl/min and 56.7 nl/min | 0.0072 without wells, reduced about 16-fold with wells | 101 |
| Neural progenitors | L: 10 mm, W: 4 mm, H: 250 μm | 0.15 μl/h | NA | 102 |
| Mouse fibroblast | W: 2.8 mm, Outer D:7.6 mm, Inner D: 2 mm inner, H: 100 μm | 0.28 μl/min | 0.0004–0.0016 | 103 |
| Neonatal Schwann cells | 0.4 ml | 1 ml/min | NA | 127 |