Skip to main content
. 2013 Nov 15;8(11):e80617. doi: 10.1371/journal.pone.0080617

Figure 6. Cul3 controls adenovirus early E1A gene expression in infected cells.

Figure 6

(A) DN-Cul3 reduces expression of the Ad5 E1A protein. Expression of the DN-Cul3-Flag protein was induced with Dox in HeLa-DN-Cul3-Flag stable cell line for 24 hours followed by Ad5 infection (MOI=5). Total cell lysates were prepared 6 hpi, 12 hpi and 24 hpi and the expression levels of the viral E1A, viral capsid proteins, Flag-tagged DN-Cul3 and Actin proteins were detected by Western blotting. Black arrow indicates the migration of the DN-Cul3-Flag protein, asterisk (*) indicates a non-specific protein detected with anti-Flag antibody (Sigma, F7425). Representative image from three independent experiments is shown. (B) DN-Cul3 reduces Ad5 E1A mRNA accumulation. E1A mRNA expression was detected by qRT-PCR from the HeLa-DN-Cul3-Flag stable cell lines undergoing the same treatments as in (A). Data is shown as the average from two independent experiments. E1A expression levels were normalized to the 18S rRNA levels. Hour post infection (hpi).