Table 1. Immunogenic proteins observed in B. cenocepacia strains as identified by 2-D gels and MALDI-ToF mass spectrometry.
| Protein name | Functional category | Accession Number | Predicted Subcellular locationa | Localisation score | MW(kDa) /pIb | Seq. coverage (%) | Scorec | Strain |
|---|---|---|---|---|---|---|---|---|
| alkyl hydroperoxide reductase/ Thiol specific antioxidant | Cellular processes | gi|107028881 | CP | 9.97 | 20.5/4.9 | 44-55 | 84-118 | BC-7, C1394 |
| chaperonin GroEL | Protein stabilisation | gi|161525697 | CP | 9.97 | 57.1/5.0 | 46-66 | 200-217 | BC-7, C1394 |
| dipeptide transporter ATP-binding subunit | Transport and binding | gi|107023972 | CPM | 7.88 | 37.5/9.5 | 26.3-42 | 63-107 | BC-7, C1394 |
| divergent AAA domain protein | Transcription | gi|323143202 | CP | 8.96 | 57.1/7 | 21-27 | 86-92 | BC-7, C1394 |
| DNA-directed RNA polymerase subunit alpha | Transcription | gi|161523455 | CP | 9.97 | 35.7/5.6 | 43-60 | 81-172 | BC-7, C1394 |
| elongation factor Tu | Transcription | gi|78064909 gi|221213513 | CP | 9.97 | 43.1/5.3 | 27-61 | 52-113 | BC-7, C1394 |
| phosphopyruvate hydratase | Energy metabolism - glycolysis | gi|134296288 | CP | 9.97 | 45.9/4.6 | 46-50 | 139-119 | BC-7, C1394 |
| porin | Transport and binding protein | gi|107025986 | OM | 10.0 | 37.7/9.7 | 40-65 | 119-139 | BC-7, C1394 |
| putative ubiquinone biosynthesis protein UbiB | Unknown function | gi|161523750 | CPM | 7.88 | 59.9/9.3 | 21.7-22 | 66 | BC-7, C1394 |
| 30S ribosomal protein S1 | Protein synthesis | gi|161525427 | CP | 9.97 | 62.2/4.9 | 22-26 | 88-110 | BC-7 |
| Enoyl CoA hydratase | Cell-cell communication | gi|311104936 | CP | 9.97 | 29.8/4.8 | 32-34 | 102-121 | BC-7 |
| EvpB family type VI secretion protein | Secretion protein | gi|161526098 | CP | 9.26 | 55.0/5.1 | 52-57 | 91-97 | BC-7 |
| F0F1 ATP synthase subunit alpha | Energy metabolism - electron motive force | gi|161523284 | CP | 9.97 | 55.8/5.5 | 32.7-62.1 | 96-185 | BC-7 |
| Hypothetical protein BCAS0292 | Unknown function | gi|197295141 | U | U | 19.9/5.6 | 56-84 | 180-208 | BC7 |
| LysM domain/M23 peptidase domain protein | Peptidoglycan binding | gi|221198090 | OM | 9.93 | 30.0/9.9 | 37-44 | 66-83 | BC-7 |
| OmpA/MotB domain-containing protein | OM protein | gi|115350969 | OM | 9.93 | 24.1/10.1 | 55-56 | 105-328 | BC-7 |
| outer membrane efflux protein OprA | Transport and binding | gi|221212959 | OM | 10.0 | 52.9/9.1 | 37-44 | 66-83 | BC-7 |
| outer membrane protein OprM | Transport and binding | gi|221200195 | OM | 10.0 | 54.3/5/7 | 36.9-43 | 112-524 | BC-7 |
| oxidoreductase, aldo/keto reductase family | Energy metabolism | gi|221201924 | CP | 9.97 | 38.2/5.8 | 44-56.8 | 95-114 | BC-7 |
| Peptidoglycan-associated lipoprotein | Transport | gi|357936457 | OM | 10.0 | 18.5/6.6 | 23-30 | 58 | BC-7 |
| Polyribonucleotide nucleotidyltransferase | RNA binding and catabolic processing | gi|221211731 | CP | 9.97 | 77/5.2 | 24.9-28 | 94-111 | BC-7 |
| Putative ferritin DPS-family Binding protein | DNA binding | gi|206561636 | CP | 9.26 | 18.0/5.7 | 46-56 | 100-124 | BC-7 |
| putative outer membrane porin | Transport and binding protein | gi|221196031 | OM | 10.0 | 38.0/9.5 | 39.0-49.9 | 109-685 | BC-7 |
| ubiquinone/menaquinone biosynthesis methyltransferase | Metabolic processes | gi|107023667 | CP | 9.97 | 27.1/9.1 | 30-54 | 81-130 | BC-7 |
| acetyl-CoA acetyltransferase | Metabolic processes | gi|107023705 | CP | 9.97 | 40.5/6.7 | 19-51 | 100-273 | C1394 |
| Zn-dependent alcohol dehydrogenase | Energy metabolism glycolysis, gluconeogenesis, chloroalkane metabolism | gi|254248364 | CP | 9.97 | 38.2/6.3 | 52-57 | 102-204 | C1394 |
| aldehyde dehydrogenase | Energy metabolism / glycolysis, gluconeogenesis, chloroalkane metabolism | gi|170737358 | CP | 9.97 | 54.2/5.8 | 20.5-34.2 | 71-102 | C1394 |
| cell shape determining protein, mreb/mrl family | Cell membrane | gi|319761104 | CP | 9.97 | 37.0/5.1 | 24 | 82-94 | C1394 |
| F0F1 ATP synthase subunit beta | Energy metabolism - ATP-proton motive force interconversion | gi|107024491 | CP | 9.12 | 50.7/5.1 | 34-40 | 82-104 | C1394 |
| HAD family hydrolase | Unknown function/ enzyme of unknown specificity | gi|107022869 | CP | 9.97 | 24.5/5.3 | 48.8-57 | 66-101 | C1394 |
| hypothetical protein Bcen_2492 | Hypothetical protein | gi|107024037 | CP | 9.97 | 32.0/8.9 | 32-46 | 71-140 | C1394 |
| inosine 5'-monophosphate dehydrogenase | Unknown function/ enzyme of unknown specificity | gi|206560426 gi|115352075 | CP | 9.97 | 52.2/7.1 | 32.3-51.4 | 80-150 | C1394 |
| Thiolase | Unknown function/ enzyme of unknown specificity | gi|254248092 | CP | 9.97 | 40.5/7.8 | 37.9-57.1 | 98-196 | C1394 |
a Predicted subcellular localisation determined using PSORTb V3 (www.psort.org) [19]. OM: outer membrane; CPM: Cytoplasmic membrane; CP: cytoplasmic, U: unknown.
b Theoretical molecular mass (kDa) and isoelectric point were determined by Mascot.
c Ranges represent MS/MS ion scores determined by peptide mass fingerprinting [61]. Only scores which were deemed to be significant by Mascot (p<0.05) are reported.