Table 2. Immunogenic proteins observed in B. multivorans strains as identified by 2-D gels and MALDI-Tof mass spectrometry.
| Protein name | Functional category | Accession Number | Subcellular location a | Localisation score | MW(kDa)/pIb | Seq. coverage (%) | Scorec | Strain |
|---|---|---|---|---|---|---|---|---|
| 30S ribosomal protein S1 | Protein synthesis | gi|161525427 | CP | 9.97 | 62.2/4.9 | 32.1-39 | 132-160 | LMG13010, C1962 |
| cell shape determining protein, mreb/mrl family | Cell envelope | gi|319761104 | CP | 9.97 | 37.0/5.7 | 26-34 | 92-128 | LMG13010, C1962 |
| chaperonin GroEL | Protein stabilisation | gi|161525697 | CP | 9.97 | 57.1/5 | 24-65.5 | 111-310 | LMG13010, C1962 |
| DNA-directed RNA polymerase subunit alpha | Transcription | gi|161523455 | CP | 9.97 | 35.8/ 5.6 | 41.8 -63.4 | 167-235 | LMG13010, C1962 |
| elongation factor Tu | Transcription | gi|221213513 | CP | 9.97 | 41.8/5 | 37.9-49 | 96-122 | LMG13010, C1962 |
| F0F1 ATP synthase subunit alpha | Energy metabolism - electron motive force | gi|161523284 | CP | 9.97 | 55.8/5.5 | 32.7-62.1 | 96-185 | LMG13010, C1962 |
| Hypothetical protein 0835 | Hypothetical protein | gi|161524014 | P | 9.84 | 25.6/9.6 | 80-89 | 28.5-68.1 | LMG13010, C1962 |
| isocitrate lyase | Energy metabolism - Glyoxylate cycle | gi|161524402 | CP | 9.97 | 47.9/5.7 | 27.1-51.4 | 71-133 | LMG13010, C1962 |
| LysM domain/M23 peptidase domain protein | Peptidoglycan binding protein | gi|221198090 | OM | 9.93 | 30/9.9 | 40.7-46 | 71-124 | LMG13010, C1962 |
| nitrate reductase, beta subunit | Energy metabolism / anaerobic | gi|221198557 | CPM | 9.82 | 59/5.8 | 26.8-36 | 79-127 | LMG13010, C1962 |
| OmpA/MotB domain-containing protein | Outer membrane protein | gi|115350969 | OM | 9.93 | 24.1/10.1 | 34-49.1 | 84-408 | LMG13010, C1962 |
| outer membrane protein OprM | Transport and binding | gi|221200195 | OM | 10.0 | 54.3/5.7 | 26.9-41.4 | 121-245 | LMG13010, C1962 |
| oxidoreductase, aldo/keto reductase family | Energy metabolism | gi|221201924 | CP | 9.97 | 38.2/5.8 | 52.0-78.7 | 114-225 | LMG13010, C1962 |
| porin | Transport and binding | gi|161520486 | OM | 9.95 | 38.1/9.5 | 45-68.6 | 124-241 | LMG13010, C1962 |
| putative outer membrane porin | Transport and binding | gi|221196031 | OM | 10.0 | 38./9.5 | 42-55.4 | 105-434 | LMG13010, C1962 |
| soluble lytic murein transglycosylase | Cellular processes | gi|294634704 | U | U | 72.6/9.9 | 25-26 | 94-97 | LMG13010, C1962 |
| type II citrate synthase | Energy metabolism | gi|161521186 | CP | 9.97 | 48.9/6.3 | 30.7-34.5 | 77-129 | LMG13010, C1962 |
| dipeptide transporter ATP-binding subunit | Transport and binding | gi|107023972 | CPM | 7.88 | 37.5/9.5 | 36-44 | 84-98 | LMG 13010 |
| EvpB family type VI secretion protein | Secretion system | gi|161526098 | CP | 9.26 | 55/5.1 | 41.7-68 | 118-198 | LMG 13010 |
| GTP cyclohydrolase I | Haemolysis | gi|161521826 | CP | 9.97 | 23.5/9.0 | 35-67 | 81-147 | LMG13010 |
| Hcp type VI secretion system effector | Secretion system | gi|161526097 | EC | 10.0 | 18.5/6.9 | 77-82 | 99-154 | LMG13010 |
| Heat shock protein Hsp20 | Protein stabilisation | gi|161525226 | CP | 8.96 | 15.1/4.9 | 58-89 | 96-205 | LMG13010 |
| Hydroperoxide reductase | Cellular processes | gi|53719707 | CP | 9.97 | 20.4/4.9 | 44-54 | 92-154 | LMG13010 |
| Outer membrane lipoprotein precursor | Transport | gi|30314414 | OM | 10.0 | 16.5/5.7 | 50 | 87 | LMG13010 |
| Peptidoglycan binding LysM | Peptidoglycan binding | gi|221198154 | U | U | 16.3/4.6 | 62-71 | 98-116 | LMG13010 |
| phosphonate-transporting ATPase | Transport binding protein | gi|319955500 | CPM | 7.88 | 27.3/5.6 | 33-43 | 85-87 | LMG 13010 |
| phosphopyruvate hydratase | Energy metabolism - glycolysis | gi|161524338 | CP | 9.97 | 45.9/4.6 | 28.3-60 | 125-168 | LMG 13010 |
| recombinase A | Gene expression | gi|161523830 | CP | 9.97 | 38.3/4/9 | 30.5-36.4 | 66-73 | LMG 13010 |
| succinate dehydrogenase flavoprotein subunit | Energy metabolism | gi|161521189 | CPM | 7.88 | 65/6.2 | 31.-37.1 | 123-146 | LMG13010 |
| Type VI secretion protein | Secretion system | gi|161526099 | CP | 9.97 | 19.2/5.1 | 46-61 | 110-141 | LMG13010 |
| Acetyl CoA Acetyltransferase | Cell metabolism | gi|221198056 | CP | 9.97 | 40.7/7.8 | 40.2-49.9 | 78-109 | C1962 |
| Dihydrolipoyl dehydrogenase | Fatty acid synthesis | gi|221214787 | CP | 9.97 | 51.9/5.3 | 31.9-39 | 98-103 | C1962 |
| Dyp-type peroxidase family | Unknown function/ enzyme of unknown specificity | gi|221207824 | CP | 8.96 | 37.2/4.8 | 23-61 | 114-246 | C1962 |
a Predicted subcellular localisation determined using PSORTb V3 (www.psort.org) [19]. OM: outer membrane; CPM: Cytoplasmic membrane; CP: cytoplasmic; P: Periplasmic; U: unknown
b Theoretical molecular mass (kDa) and isoelectric point were determined by Mascot.
c Ranges represent MS/MS ion scores determined by peptide mass fingerprinting [61]. Only scores which were deemed to be significant by Mascot (p<0.05) are reported.