Table 1. Primers used for cloning of IL12-SS1 (Fv).
| Primers | Note | Sequences |
|---|---|---|
| 9228 | p40 Forward | 5’-ATGTGGGAGCTGGAGAAAGACGTTTATG-3’ |
| 9229 | p35 Forward | 5’-CCGATCCGGTGGCGGTGGCTCGGGCGGTGGTGGGTC GGGTGGCGGCGGATCTAGGGTCATTCCAGTCTCTGGACCTGCC-3’ |
| 9230 | p40 Reverse | 5’-CCGACCCACCACCGCCCGAGCCACCGCCACCGGATC GGACCCTGCAGGGAACACATGCCC-3’ |
| 9231 | SS1 Forward | 5’ – GGCTATCTGAGTTCCGCCGGAAGCGCTGATGGAGGT ATGGCCCAGGTGCAGCTGCAGCAG-3’ |
| 9233 | SS1 Reverse | 5’-GCCCTTGTCGTCATCGTCCTTATAATCGCCCCGTTTTA TTTCCAACTTTGTCCCAGC-3’ |
| 9234 | p40 Forward | 5’-CCATCTCCTGGTTTGCCATCGTTTTGCTGGTGTCTCCA CTCATGGCCATGTGGGAGCTGGAGAAAGACG-3’ |
| 9235 | p40 Reverse | 5’-GGGGACAACTTTGTACAAGAAAGTTGATTAATGGTG ATGGTGATGGTGATGGTGGCCCTTGTCGTCATCGTCC-3’ |
| 9237 | Adaptor | 5’-GGGGACAACTTTGTACAAAAAAGTTGGCACCATGTG TCCTCAGAAGCTAACCATCTCCTGGTTTGCCATCG-3’ |
| 9330 | p35 Reverse | 5’-CGCTTCCGGCGGAACTCAGATAGCCCATCACCCTGTT GATGGTCACGACGCGGGTGCTGAAGGCG-3’ |
Cloning for IL12-SS1 (Fv). IL12 p40: 9228 + 9230; IL12 p35: 9229 + 9330; scFv SS1: 9231 + 9233 and IL12-SS1 (Fv): 9234 + 9235 + 9237. Primer 9229 and 9230 contained linker (Ser4Gly)3, 9231 and 9330 contained linker GSADGG, Adaptor 9237 included attB1 sequence.