Fig. 3.

Salt tolerance of AtCSP3 overexpressors. Semi-quantitative RT-PCR (A) and immunoblot analysis (B) of AtCSP3 expression in 10-d-old seedlings. Three independent transgenic lines (S3–3, S3–29, and S3–31) were analyzed. (C) Phenotype of AtCSP3-overexpressing transgenic plants on high-salt medium (200 mM NaCl). The photograph was taken after 6 d of salt treatment. (D) Survival rates were calculated from three independent experiments (n = 25). Data represent the means ± SE. (E) Root growth of AtCSP3 transgenic plants in response to salt treatment (125 mM NaCl). The picture was taken 7 d after transfer to salt-supplemented medium. (F) Relative root length to plants without the salt stress was calculated from three independent experiments (n = 5). The data represent the means ± SE. Asterisks in (D) and (F) indicate significantly higher survival rates than wild-type (WT) plants as determined by Student's t test (**P < 0.01).