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PCR oligonucleotide primers used in this study.
| Mutant | Forward primer | Reverse primer |
|---|---|---|
| Q193R | GAAACGACTTGGATCACACGTCACGAGCCGGCCTTTCTGGC | GCCAGAAAGGCCGGCTCGTGACGTGTGATCCAAGTCGTTTC |
| H194T | GACTTGGATCACACAGACCGAGCCGGCCTTTCTGG | CCAGAAAGGCCGGCTCGGTCTGTGTGATCCAAGTC |
| N173X | GCAATTATCGGTGGCGGTNDTTCTGGCGCACAG | n/a |
| S174X | CGGTGGCGGTAATNDTGGCGCACAGATCCTGGC | n/a |
| F52X | GGCATTCTCTGCATCTGNDTAGCCCAGCGGGCTGG | n/a |
