Table 2.

ELISA assay technical specifications. Specifications of the competitive ELISA assays, IC6 and C6M, utilized in this study

	IC6	C6M
Coater peptide	Biotin-ADWGQSRDAEEIAISQ	YRGPEGPQGP-K-Biotin
Coater buffer	25 mM PBS-BTB pH 7.4	25 mM Tris/BTB, 1 % BSA, 0.1 % Tween-20, pH 8.0
Ab incubation buffer	25 mM PBS-BTB pH 7.4	25 mM Tris/BTB, 1 % BSA, 0.1 % Tween-20, +5 % Liquid II, pH 8.0
Ab incubation conditions	20 h at 4 °C	1 h at 20 °C on a shaker at 300 rpm
Standard/calibrator concentration range	20–0.3 ng/ml	242.6–0.5 ng/ml