Fig. 1. IL-1β increased Tgase-2 and OPG expression in MG-63 osteosarcoma cells. (A) IL-1β time-dependently induced OPG and Tgase-2 expression in cell lysates. MG-63 cells were treated with control media or IL-1β (5 ng/ml) for indicated time. Whole-cell lysates were subjected to 4-12% SDS-PAGE, and expression of Tgase-2 and OPG were determined by Western blotting. β-actin was used here as an internal control. (B) IL-1β time-dependently induced 110 kDa OPG expressions in the cell media. Culture media were subjected to 4-12% SDS-PAGE, and the expression of OPG was determined by Western blotting.
