Fig. 2. TPA increased Tgase-2 and OPG expression in MG-63 osteosarcoma cells. (A) TPA induced OPG and Tgase-2 expression in cell lysates. MG-63 cells were treated with control media or TPA (10 nM) for the indicated time. Whole-cell lysates were subjected to 4-12% SDS-PAGE, and expression of Tgase-2 and OPG were determined by Western blotting. β-actin was used here as an internal control. (B) TPA induced OPG expressions in the cell media. Culture mediums were subjected to 4-12% SDS-PAGE, and expression of OPG was determined by Western blotting.
