Fig. 4. Expression of N-epsilon gamma glutamyl lysine in IL-1β stimulated MG-63 osteosarcoma. MG-63 cells were treated with the control media or IL-1β (5 ng/ml) for the indicated time. Conditional media were prepared and the protein level was subjected to 4-12% SDS-PAGE, and expression of N-epsilon gamma glutamyl lysine was determined by Western blotting.
