Fig. 3. Effects of aromadendrin on LPS-induced iNOS and COX-2 protein (A) and mRNA (B) expressions in RAW 264.7 cells. (A) The cell lysates were subjected to SDS-PAGE, and then protein levels of iNOS and COX-2 were determined by Western blot analysis. Aromadendrin significantly attenuated LPS-induced overexpression of iNOS and COX-2. (B) After LPS treatment for 6 hr, total mRNA levels of iNOS and COX-2 were examined by RT-PCR. GAPDH was used as an internal control. Aromadendrin significantly suppressed LPS-induced iNOS and COX-2 expression in concentration-dependent manners. Images are representative of three independent experiments that shows reproducible results. ARD stands for aromadendrin.